Composite

Part:BBa_K2541404:Design

Designed by: Shuting Zheng   Group: iGEM18_Jilin_China   (2018-10-05)


sfGFP(BbsI free) measurement device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

The sfGFP(BbsI free) is BbsI restriction site free and can be used in Golden Gate assembly.

Source

sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)- from 5’-gaagac-3’ to 5’-gaggat-3’. And other sequences are derived from Registry of Standard Biological Parts.

References

[1]Southward CM, Surette MG. 2002. The dynamic microbe: green fluorescent protein brings bacteria to light. Mol. Microbiol. 45:1191–1196.

[2]Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38.

[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88.

[4]Overkamp W, Beilharz K, Detert O W R, et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.[J]. Applied & Environmental Microbiology, 2013, 79(20):6481-6490.